Significance of 'heat-shock' method in bacterial transformations is to facilitate:

1

(a)

Binding of DNA to the cell wall

(b)

Uptake of DNA through membrane transport proteins

(c)

Uptake of DNA through transient pores in the bacterial cell wall

(d)

Expression of antibiotic resistance gene

The role of DNA ligase in the construction of a recombinant DNS molecule is:

1

(a)

Formation of phosphodiester bond between two DNA fragments

(b)

Formation of hydrogen bonds between sticky ends of DNA fragments

(c)

Ligation of all purine and pyrimidine bases

(d)

None of the above

Which of the following bacteria is not a source of restriction endonuclease?

1

(a)

Haemophilus influenzae

(b)

Escherichia coli

(c)

Agrobacterium tumefaciens

(d)

Bacillus amyloli

Which of the following steps are catalysed by Taq polymerase in a PCR reaction?

1

(a)

Denaturation of template DNA

(b)

Annealing of primers to template DNA

(c)

Extension of primer end on the template DNA

(d)

All of the above

Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?

1

(a)

Laboratory flask of largest capacity

(b)

A stirred-tank bioreactor without in-lets and out-lets

(c)

A continuous culture system

(d)

Any of the above

Who among the following was awarded the Nobel Prize for the development of PCR technique?

1

(a)

Herbert Boyer

(b)

Hargovind Khurana

(c)

Kary Mullis

(d)

Arthur Kornberg

Restriction enzyme Eco RI cuts the DNA between bases G and A only when sequence in DNA is

1

(a)

GATATC

(b)

GAATTC

(c)

GATTCC

(d)

GAACTT

While isolating DNA from bacteria, which of the following enzymes is not used?

1

(a)

Lysozyme

(b)

Ribonuclease

(c)

Deoxyribonuclease

(d)

Protease

Which of the given statement is correct in the context of observing DNA separated by agarose gel electrophoresis?

1

(a)

DNA can be seen in visible light

(b)

DNA can be seen without staining in visible light

(c)

Ethidium bromide stained DNA can be seen in visible light

(d)

Ethidium bromide stained DNA can be seen under exposure to UV light

'Restriction' in Restriction enzyme refers to

1

(a)

cleaving of phosphodiester bond in DNA by the enzyme

(b)

cutting of DNA at specific position only

(c)

prevention ofthe multiplication of bacteriophage in bacteria

(d)

all of the above