Totipotency refers to _____.

(a)

capacity to generate genetically identical plants.

(b)

capacity to generate a whole plant from any plant cell / explant.

(c)

capacity to generate hybrid protoplasts.

(d)

recovery of healthy plants from diseased plants.

The time duration for sterilization process by using autoclave is ______ minutes and the temperature is ______.

(a)

10 to 30 minutes and 125° C

(b)

15 to 30 minutes and 121° C

(c)

15 to 20 minutes and 125° C

(d)

10 to 20 minutes and 121° C

Virus free plants are developed from _____.

(a)

Organ culture

(b)

Meristem culture

(c)

Protoplast culture

(d)

Cell suspension culture

The prevention of large scale loss of biological integrity ____.

(a)

Biopatent

(b)

Bioethics

(c)

Biosafety

(d)

Biofuel

Solidifying agent used in plant tissue culture is _____.

(a)

Nicotinic acid

(b)

Cobaltous chloride

(c)

EDTA

(d)

Agar

The production of secondary metabolites require the use of _________.

(a)

Protoplast culture

(b)

Organ culture

(c)

Cell suspension culture

(d)

Virus free germ culture

Solidifying agent

(1)

Sucrose

Cryoprotectant

(2)

IAA

Growth hormone

(3)

Agar

Fusogen

(4)

Agar

Write the various steps involved in cell suspension culture.

What do you mean Embryoids? Write its application.

Define tissue culture.

Define sterilization.

What is a cybrid?

Compare Redifferentiation with Dedifferentiation.

How cell suspension is prepared?

Name any three secondary metabolites obtained from plants and mention their medicinal aspects.

Comment on Biosafety.

Name the explant through which virus free plantlets can be generated using tissue culturing technique.

Give an account on Cryopreservation.

Write the protocol for artificial seed preparation.

Explain the steps involved in protoplast culture.