Micro propagation involves _____.

(a)

vegetative multiplication of plants by using micro-organisms.

(b)

vegetative multiplication of plants by using small explants.

(c)

vegetative multiplication of plants by using microspores.

(d)

Non-vegetative multiplication of plants by using microspores and megaspores.

The time duration for sterilization process by using autoclave is ______ minutes and the temperature is ______.

(a)

10 to 30 minutes and 125° C

(b)

15 to 30 minutes and 121° C

(c)

15 to 20 minutes and 125° C

(d)

10 to 20 minutes and 121° C

Virus free plants are developed from _____.

(a)

Organ culture

(b)

Meristem culture

(c)

Protoplast culture

(d)

Cell suspension culture

The prevention of large scale loss of biological interity ____.

(a)

Biopatent

(b)

Bioethics

(c)

Biosafety

(d)

Biofuel

Identify the group of scientists who developed the intergenic hybrid - the pomato.

(a)

Yamada et al.

(b)

Horsh et al.

(c)

Takebe et al.

(d)

Melchers et al.

A widely used fusogen in protoplast culture is ________

(a)

Polymethyl glycol

(b)

Polyethylene glycol

(c)

Polyethylene chloride

(d)

Polyvinyl chloride

Solidifying agent

(1)

IAA

Cryoprotectant

(2)

Sucrose

Growth hormone

(3)

Agar

Fusogen

(4)

Agar

How will you avoid the growing of microbes in nutrient medium during culture process? What are the techniques used to remove the microbes?

Write the various steps involved in cell suspension culture.

What do you mean Embryoids? Write its application.

Define tissue culture.

Define sterilization.

Name any four culture media used in plant tissue culture.

Give the examples for micro propagation performed plants .

Explain the basic concepts involved in plant tissue culture

Compare Redifferentiation with Dedifferentiation.

Mention any three macronutrients and micro nutrients used in MS medium.

Name any three secondary metabolites obtained from plants and mention their medicinal aspects.

What is cybrid?

Give an account on Cryopreservation.

Write the protocol for artificial seed preparation.