Plasmids are ______.

(a)

circular protein molecules

(b)

required by bacteria

(c)

tiny bacteria

(d)

confer resistance to antibiotics

An analysis of chromosomal DNA using the southern hybridisation technique does not use _____.

(a)

Electrophoresis

(b)

Blotting

(c)

Autoradiography

(d)

Polymerase Chain Reaction

Zymology deals with __________

(a)

Study of yeast fungus and its practical applications.

(b)

Study of fermentation and its uses.

(c)

Study of Bioreactors and their construction methodology.

(d)

Study of zymase producing microbes and its benefits.

The bacteria responsible for causing Crown Gall:

(a)

Escherichia coli

(b)

Bacillus subtilis

(c)

Bacillus thuringiensis

(d)

Agrobacterium tumefaciens

Statement 1: DNA is a hydrophobic molecule.
Statement 2: T-DNA is a part of E-coli plasmid.

(a)

Statement 1 is correct and Statement 2 is also correct.

(b)

Statement 1 is correct and Statement 2 is incorrect.

(c)

Both the statements are incorrect.

(d)

Statement 1 is incorrect and Statement 2 is correct.

Assertion (A) : Pseudomonas putida is utilized in the production of Biological hydrogen.
Reason (R): During photosynthesis, it releases oxygen.

(a)

Both A and R are wrong.

(b)

A is right R is wrong.

(c)

R explains A.

(d)

A and R are right, R does not explain A.

Assertion (A): DMH -11 is a transgenic mustard.
Reason (R): It is developed by using barnase/ barstar technology.

(a)

Both A and R are wrong.

(b)

A is right R is wrong.

(c)

R explains A.

(d)

A and R are right, R does not explain A.

________is the plasmid present in Agrobacterium

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Ti plasmid

What are the enzymes you can used to cut terminal end and internal phospho di ester bond of nucleotide sequence?

How microbial enzymes are produced? Mention its significance.

Classify vectors and explain them.

Give the technical terminologies for the following statements.
(a) Autonomous, self-replicating, circular DNA
(b) Molecular scissors
(c) Symmetrical repeated sequence in DNA strands
(d) Mobile genetic elements

What are restriction enzyme. Mention their type with role in Biotechnology.

Which type of restriction enzyme is widely used in rDNA technology? Why?

Why is it difficult for DNA to pass through cell membrane? How the bacterial cells can be made competent to take up DNA?

What is bioremediation? give some examples of bioremediation.

Explain Agarose Gel electrophoresis technique.