Dolly, the sheep was obtained by a technique known as

(a)

Cloning by gene transfer

(b)

Cloning without the help of gametes

(c)

Cloning by tissue culture of somatic cells

(d)

Cloning by nuclear transfer

Transgenic animals are those which have

(a)

Foreign DNA in some of their cells

(b)

Foreign DNA in all their cells

(c)

Foreign RNA in some of their cells

(d)

Foreign RNA in all their cells

Recombinant Factor VIII is produced in the ______ cells of the Chinese Hamster

(a)

Liver cells

(b)

blood cells

(c)

ovarian cells

(d)

brain cells.

Vaccines that use components of a pathogenic organism rather than the whole organism are called

(a)

Subunit recombinant vaccines

(b)

attenuated recombinant vaccines

(c)

DNA vaccines

(d)

conventional vaccines

Interferons are produced using________

(a)

Pigs

(b)

yeast

(c)

E.coli

(d)

viruses

Choose the option not applicable to ELISA

(a)

It is a bio chemical produce to detect antigens in a sample

(b)

The intensity of colour obtained in the test is proportional to amount of antigen.

(c)

It can detect DNA

(d)

ELISA is a test for detecting human chorionic gonadotropin hormone

The genes for the formation of the blood dotting factor VIII is located in the_______chromosome.

(a)

autosome

(b)

X chromosome

(c)

allosome

(d)

Y chromosome

In the geom line gene therapy, the genes are introduced into the______

(a)

bone marrow cells

(b)

blood cells

(c)

sperms and eggs

(d)

skin cells

This type of gene therapy involves the insulin C the anti sense gene which inhibits the expression of the dominant gene

(a)

gene inhibition therapy

(b)

gene augmentation therapy

(c)

germ line gene therapy

(d)

somatic cell gene therapy

___________among the following will not serve as store house of stem cell.

(a)

Blood

(b)

Bone marrow

(c)

Amniotic fluid

(d)

Placenta

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

(a)

5' - CGTTCG - 3' 3' -ATGGTA -5'

(b)

5'-GATATG -3' 3' CTACTA -5'

(c)

5' -GAATTC - 3' 3' - CTTAAG-5'

(d)

5' -CACGTA -3' 3' -CTCAGT -5'

Identify the incorrect statement.
(i) The first clinical gene therapy was given by French Anderson.
(ii) For a four year old boy with ADA deficiency.
(iii) ACD is a autosomal dominant metabolic disorder.
(iv) Where patients have non-functioning B -lymphocytes.

(a)

i and iv only

(b)

ii, iii and iv

(c)

i, ii and iv

(d)

all the above

PCR technique was developed by

(a)

Eva Engvall

(b)

Peter Perlmann

(c)

Kary Mullis

(d)

Wilmut

Arrange the steps of PCR in proper sequence

(a)

Denaturation, Primer extension, Renaturation

(b)

Renaturation, Denaturation, Primer extension

(c)

Primer extension, Denaturation, Renaturati

(d)

Denaturation, Renaturation, Primer extension

In cloning process of Dolly, how many embryos were implemented by Ian Wilmut and Campbell, out of which one successful Dolly was developed?

(a)

267

(b)

277

(c)

287

(d)

307

Gene therapy is an attempt to correct a Genetic defect by providing a normal gene into the individual. By this the function can be restored. An alternate method would be to provide gene product known as enzyme replacement therapy, which would also restore the function. Which in your opinion is a better option? Give reasons for your answer.

What is hGH?

Name the types of recombinant vaccines.

What is a patent?

How insulin controls blood sugar level?

Name the two strategies involved in gene therapy

Comment on SCID

What does ELISA stands for? Who invented this technique?

Name the various kinds of ELISA.

Simply define the PCR technique. Also mention its inventor

What are transgenic animals? Give examples

What are DNA vaccines?

Differentiate between Somatic cell gene therapy and germline gene therapy

One of the applications of biotechnology is ‘gene therapy” to treat a person born with a hereditary disease
i) What does “gene therapy” mean?
ii) Name the hereditary disease for which the first clinical gene therapy was used.
iii) Mention the steps involved in gene therapy to treat this disease.

Why is E.Coli not used for production of Interferons?

Can DNA vaccine cause the disease?

Briefly explain the structure of insulin

State any two uniqueness of ELISA test.

Saccharomyces cerevisiae, acts as a best host than Encherichia coli for the production of recombinant interferons. Yes or No? Support your answer.

Functional Insulin differs from its pre-hormonal form. How?

PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate

Mention the advantages (3) and disadvantages of cloning (3).

Explain the steps involved in the production of recombinant hGH

LIst the uses of transgenesis

Explain in detail about stem cell therapy. (or) What are the multipotent cells involved in replenishing adult tissue ? What is the rich source for it?

Enumerate the steps involved in producing transgenic animals.

Describe the procedure by which Dolly was developed.