Which one of the following statements is true regarding DNA polymerase used in PCR?

(a)

It is used to ligate introduced DNA in recipient cells

(b)

It serves as a selectable marker

(c)

It is isolated from a Virus

(d)

It remains active at a high temperature

ELISA is mainly used for

(a)

Detection of mutations

(b)

Detection of pathogens

(c)

Selecting animals having desired traits

(d)

Selecting plants having desired traits

Vaccines that use components of a pathogenic organism rather than the whole organism are called

(a)

Subunit recombinant vaccines

(b)

attenuated recombinant vaccines

(c)

DNA vaccines

(d)

conventional vaccines

Insulin was first isolated by_______

(a)

Banting

(b)

Allen

(c)

Wilmut

(d)

Anderson

Best and Banting isolated insulin from pancreatic islets of___________

(a)

E.coli

(b)

yeast

(c)

dog

(d)

pig

Interferons were discovered by________

(a)

Banting

(b)

Flemming

(c)

Engvall

(d)

Alick Isaacs and Lindemann

The genes for the formation of the blood dotting factor VIII is located in the_______chromosome.

(a)

autosome

(b)

X chromosome

(c)

allosome

(d)

Y chromosome

In the geom line gene therapy, the genes are introduced into the______

(a)

bone marrow cells

(b)

blood cells

(c)

sperms and eggs

(d)

skin cells

This type of gene therapy involves the insulin C the anti sense gene which inhibits the expression of the dominant gene

(a)

gene inhibition therapy

(b)

gene augmentation therapy

(c)

germ line gene therapy

(d)

somatic cell gene therapy

The purpose of using enzyme in the ELISA test is

(a)

to show the coloured product

(b)

to show the antigen-antibody reaction

(c)

to show the immobilised antigen

(d)

to show the immobilized antigen

Through PCR technique, amplication of_________ is possible with reverse transcriptase

(a)

DNA

(b)

rRNA

(c)

tRNA

(d)

mRNA

________was the first GI tagged product in India

(a)

Kancheepuram silk

(b)

Darjeeling tea

(c)

Thanjavur paintings

(d)

Temple jewellery of Nagarcoil

In genetic engineering, a DNS segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A to D) in this regard and select correct option about which one or more of these can be used as vector/vectors.
A) A bacterium
B) Plasmid
C) Plasmodium
D) Bacteriophage

(a)

(A), (B) and (D) only

(b)

(A) only

(c)

(A) and (C) only

(d)

(B) and (D) only

The first transgenic clone of sheep was called as_________ 

(a)

Rosie

(b)

Dolly

(c)

Sameera

(d)

Joel

Name the scientists who discovered Interferons?

(a)

Alick Issac and Jean Lindemann

(b)

Thalassemia

(c)

Haemophilia

(d)

Anaemia

Describe the chemical structure of insulin

What is alpha lactalbumin?

Define totipotency.

What is annealing?

Give examples of diseases which can be detected by PCR.

What is BRAI?

Name the two strategies involved in gene therapy

Simply define the PCR technique. Also mention its inventor

What are the Genetically Modified Organisms?

Define Biotechnology.

What are transgenic animals? Give examples

What are DNA vaccines?

Differentiate between Somatic cell gene therapy and germline gene therapy

One of the applications of biotechnology is ‘gene therapy” to treat a person born with a hereditary disease
i) What does “gene therapy” mean?
ii) Name the hereditary disease for which the first clinical gene therapy was used.
iii) Mention the steps involved in gene therapy to treat this disease.

What is gene augmentation therapy?

Briefly explain the structure of insulin

What is ELISA test?

The immune system of a person is suppressed, In ELISA test, the result is positive
(i) Name the disease associated with this.
(ii) Why did he loose his immunity?

Why do children cured by enzyme replacement therapy for ADA deficiency need periodic treatment? Suggest a permanent solution for this issue.

Whether PCR can be done for RNA molecules? Explain.

Mention the advantages (3) and disadvantages of cloning (3).

Explain the steps involved in the production of recombinant hGH

Explain the creation of Dolly

Explain in detail about stem cell therapy. (or) What are the multipotent cells involved in replenishing adult tissue ? What is the rich source for it?

List out the uses of Transgenesis

What are the ethical issues about cloning.