Restriction enzymes are _____.

(a)

Not always required in genetic engineering

(b)

Essential tools in genetic engineering

(c)

Nucleases that cleave DNA at specific sites

(d)

both b and c

Plasmids are ______.

(a)

circular protein molecules

(b)

required by bacteria

(c)

tiny bacteria

(d)

confer resistance to antibiotics

EcoRI cleaves DNA at ____.

(a)

AGGGTT

(b)

GTATATC

(c)

GAATTC

(d)

TATAGC

Genetic engineering is _____.

(a)

making artificial genes.

(b)

hybridization of DNA of one organism to that of the others.

(c)

production of alcohol by using micro organisms.

(d)

making artificial limbs, diagnostic instruments such as ECG, EEG etc.,

Consider the following statements:
I. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man invitro
II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid
III. Restriction enzymes belongs to a class of enzymes called nucleases.
Choose the correct option regarding above statements

(a)

I & II

(b)

I & III

(c)

II & III

(d)

I,II & III