How is copy number of the plasmid vector related to yield of recombinant protein?

1

(a)

The recombinant DNA can multiply as many times as the copy number of the plasmid vector. thereby determning the yield of recombinant protein So, higher the copy number of the plasmid vector, higher will be the yield of recombinant protein.

Would you choose an exonuclease for producing a recombinant DNA molecule?

1

(a)

No, because exonucleases will remove the nucleotides from the ends DNA molecule and not at any particular desired region.

What does 'competent' refer to, in competent cells used in transformation experiment?

1

(a)

Competent refers to the ability of the host cell to take up tha alien or recombinant DNA

What is the significance of adding proteases at the time of isolation of genetic material (DNA)?

1

(a)

Proteases remove the proteins associated with the DNA.

Name a recombinant vaccine that is currently being used in vaccination pro-grammes

1

(a)

Hepatitis-B vaccine.

Restriction enzymes present in the cloning site of a vector should not have more than one recognition site. Comment.

1

(a)

If the restriction enzymes have more than one recognition site in a vector, than the vector itself will get fragmented on treatement with the restriction enzyme.

Do molecules (DNA, protein) exhibit biological activity in anhydrous conditions?

1

(a)

DNA and proteins show their activity only in presence of water.

Name the enzyme responsible for cleavage in the following sequence:
               \(\downarrow \)  
5' ___ G A A T T C ____3'
3' ___C T T A A G ____5'
                             \(\uparrow \)

1

(a)

Type II restriction enzyme obtained from Escherichia coli, called EcoRI.

Write the significance of adding proteases at the time of isolation of genetic materials.

1

(a)

Protease is added because it helps in digesting proteins and helps in the breakdown of cell wall by dissolving it.

Why do DNA fragments move towards the anode during gel electrophoresis? If yes, explain.

1

(a)

Yes, it is because DNA fragments are negatively charged. Hence, move towards the anode (positive charge).