CBSE 12th Standard Biology Subject Biotechnology: Principles and Processes HOT Questions 1 Marks Questions 2021
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CBSE 12th Standard Biology Subject Biotechnology: Principles and Processes HOT Questions 1 Marks Questions 2021
12th Standard CBSE
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Reg.No. :
Biology
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Why is the enzyme cellulase needed for isolating genetic material from plant cells and not from animal cells?
(a)Cellulase is used to digest the cell wall (cellulose) of plant cells; animal cell have no cell wall and hence, it is not needed.
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Why is it essential to have a 'selectable marker' in a cloning vector?
(a)It helps in identifying the recombinants from the non-recombinants.
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Restiction enzymes should not have mora than one site of action in the cloning site of a vector? Comment
(a)Presence of more than one recognition site will generate several segments and complicate the gene cloning.
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Would you choose an exonuclease for producing a recombinant DNA molecule?
(a)No, because exonucleases will remove the nucleotides from the ends DNA molecule and not at any particular desired region.
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Retroviruses are disease-causing microorganisms, even then are efficiently used in biotechnology experiments. Explain how is it possible?
(a)Retroviruses in which its disease-causing gene is removed (inactivated) can be used as vectors to deliver the recombinant/alien DNA into animal cells.
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Why do DNA fragments move towards the anode during gel electrophoresis? If yes, explain.
(a)Yes, it is because DNA fragments are negatively charged. Hence, move towards the anode (positive charge).
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Any DNA flanked at its two borders by T-DNA can be integrated into the plant genome. Name the vector used for this plant gene transfer?
(a)Tumor inducing plasmids (Ti Plasmids)
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In making bacteria as a competent host for transformation with r-DNA, it is treated with a specific concentration of divalent cation like calcium. What is the role of calcium?
(a)Creates pores in its cell wall
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Why are antibiotic-resistance genes used as markers in E.coli?
(a)It is because the normal E. coli cells do not have resistance to any such antibiotics.
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A plasmid without a selectable.marker was chosen as vector for cloning a gene. How does this affect the experiment?
(a)In the absence of a selectable marker, it will not be possible to identify the recombinants from the non - recombinants.
1 Marks
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CBSE 12th Standard Biology Subject Biotechnology: Principles and Processes HOT Questions 1 Marks Questions 2021 Answer Keys
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Cellulase is used to digest the cell wall (cellulose) of plant cells; animal cell have no cell wall and hence, it is not needed.
-
It helps in identifying the recombinants from the non-recombinants.
-
Presence of more than one recognition site will generate several segments and complicate the gene cloning.
-
No, because exonucleases will remove the nucleotides from the ends DNA molecule and not at any particular desired region.
-
Retroviruses in which its disease-causing gene is removed (inactivated) can be used as vectors to deliver the recombinant/alien DNA into animal cells.
-
Yes, it is because DNA fragments are negatively charged. Hence, move towards the anode (positive charge).
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Tumor inducing plasmids (Ti Plasmids)
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Creates pores in its cell wall
-
It is because the normal E. coli cells do not have resistance to any such antibiotics.
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In the absence of a selectable marker, it will not be possible to identify the recombinants from the non - recombinants.
1 Marks
