CBSE 12th Standard Biology Subject Biotechnology: Principles and Processes HOT Questions 3 Marks Questions With Solution 2021
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CBSE 12th Standard Biology Subject Biotechnology: Principles and Processes HOT Questions 3 Marks Questions With Solution 2021
12th Standard CBSE
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Reg.No. :
Biology
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Give an example of a natural form of genetic engineering in which the bacterium inserts gene into plants to cause gall or tumour formation.
(a) -
Do you see the prospects of viroids being used as plant vectors in near future?
(a) -
Give an example where a disease causing pathogen of animals has been transformed into useful vector for delevering genes to humans.
(a) -
Write the name of genus, species, strain of source bacteria from which the following endonucleases are obtained. Also write the order of their identification in the bacteria.
(i) Eco R I
(ii) Hind II(a) -
Youn have been given a task to break the cells of following organisms and release DNA along with other macromolecules from them. Name the specific enzymes you will select for the task:
(i) Bacterial cell
(ii) Plant cell
(iii) Fungal cell(a)
3 Marks
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CBSE 12th Standard Biology Subject Biotechnology: Principles and Processes HOT Questions 3 Marks Questions With Solution 2021 Answer Keys
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The best example of a natural form of genetic engineering is Agrobacterium tumefaciens inserting genes into plants and getting the plants to express those genes in the form of proteins. This infection results in the formation of a gall or tumour in the host.
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Viroids are smallest and simplest pathogenic agents comprising of 300-4000 bases long, circular, single standed, naked RNA. They are mechanically transferable and infect other parts of the plants. They have capability of being used as plant vectors
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The best example is retroviruses which have ability to transform normal animal cells into cancerous cells. These tools of pathogens are now used as vectors for delevering genes of interest to humans. These pathogens are now converted into vectors so that they do not cause any disease.
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(i)
Abbreviation of Enzyme Bacteria E Genus = Escherichia CO Species : coli R Strain : RY 13 I Ist in order of identification (ii)
Abbreviation of Enzyme Bacteria H Genus : Haemophilus in Species : influenzae d Strain : Rd II 2nd in order of identification -
(i) Lysozyme for bacterial cell
(ii) Cellulase for plant cell
(iii) Chitinase for fungal cell.
3 Marks
