CBSE 12th Standard Biology Subject Biotechnology Principles and Processes Ncert Exemplar 2 Marks Questions With Solution 2021
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CBSE 12th Standard Biology Subject Biotechnology Principles and Processes Ncert Exemplar 2 Marks Questions With Solution 2021
12th Standard CBSE
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Reg.No. :
Biology
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What is meant by gene cloning?
(a) -
You have created a recombinant DNA molecule by ligating a gene to a plasmid vector. By mistake, your friend adds exonuclease enzyme to the tube containing the recombinant DNA. How will your experiment get affected as you plan to go transformation now?
(a) -
A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?
(a) -
(i) How are recombinant vectors created?
(ii) For creating one recombinant vector only one type of restriction endonuclease is required. Give reason.(a) -
What would happen if you grow a recombinant in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
(a)
2 Marks
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CBSE 12th Standard Biology Subject Biotechnology Principles and Processes Ncert Exemplar 2 Marks Questions With Solution 2021 Answer Keys
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Formation of multiple copies of a particular gene is called gene cloning. A gene is separated and ligated to a vector like plasmid. The recombinant plasmid is introduced into a plasmid free bacterium through transformed bacterium through transformation. The transformed bacterium is made to multiply and form a colony. Each and every bacterium of the colony has a copy of the gene.
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The experiment will not likely to be affected as recombinant DNA molecule is circular closed, with no free ends. Hence, it will not be a substrate for exonuclease enzyme which removes nucleotides from the free ends of DNA.
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1) Wrong fitting of electrodes.
2) Non - use of UV = radiations.
3) Inadvert contamination with nuclease
4) Wrong fitting of electrodes. -
(i) The vector DNA is cut at a particular restriction site using a restriction enzyme ( to cut the desired DNA segment). Tha alien DNA is then linked with the plasmid DNA using an enzyme called ligase to form the recombinant vector.
(ii) Since a restriction enzyme recognizes and cuts the DNA at a particular sequence is called recognition site, the same restriction enzyme is used for cutting the DNA segment from both the vector and the other source. -
In the absence of antibiotic, there will be no pressure on recombinants to retain the plasmid (containing the gene of your interest). Since, maintaining a high copy number of plasmids is a metabolic burden to the microbial cells, it will thus, tend to lose the plasmid.
2 Marks
