CBSE 12th Standard Biology Subject Biotechnology Principles and Processes Ncert Exemplar 3 Marks Questions With Solution 2021
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CBSE 12th Standard Biology Subject Biotechnology Principles and Processes Ncert Exemplar 3 Marks Questions With Solution 2021
12th Standard CBSE
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Reg.No. :
Biology
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Mention three uses of PCR.
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Draw a labelled sketch of sparged-stirred tank bioreactor. Write its application.
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Agrobacterium tumifaciens is a soil bacterium; known as a plant pathogen as well a plasmid vector for plant transformation (genetic engineering). Enlist other bacterial plant pathogens or their relatives that have widely used in agriculture and food production.
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Bacteriophages are virus infecting bacteria, that are used as vectors because of their high copy number.
Lambda phage is a non-contractile bacteriophage used as vector that follows two life cycle, lysogenic and lytic. Explain, both the life cycles with the help of a diagram -
(i) A bacterial cell is shown in the figure given below. Label the part 'A' and 'B'. Also mention the use of part 'A' in rDNA technology.
(ii) Suppose a linear DNA fragment and a plasmid has three restriction sites for Eco RI. How many fragments will be produced from linear DNA and plasmid respectively.
5 Marks
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CBSE 12th Standard Biology Subject Biotechnology Principles and Processes Ncert Exemplar 3 Marks Questions With Solution 2021 Answer Keys
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Three uses of PCR are:
(i) It is used to during rDNA for production of newer and desired DNA
(ii) It is used for DNA sequencing.
(iii) It is used in DNA figerprinting. -
Application These bioreactors are used to produce large quantities of products enzymes, etc., using microbial, plant, animal of human cells. -
(i) Some useful strains of Agrobacterium tumefaciens (known to cause crown gall diseases) are:
(a) Agrobacterium radiabacter K84 is useful for biological control.
(b) Agrobacterium sp. M4 is a source of an experimental drug for cholesterol degradation.
(c) Agrobacterium radiobacter J14 helps in biodegradation of Atrazine, an agricultural herbicide.
(ii) Erwinia amylovora is a causative organism of fire blight disease but also serves as an elicitor of disease resistance in plants.
(iii) Xanthomonas campestris a causative organism of black rot, is also a source of xanthum gum. It is a polysaccharide used in agriculture, cosmetics and pharmaceuticals. -
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(i) In the above bacterial cell, i.e. A is plasmid B is chromosomal DNA.
(ii) If the enzyme Eco RI acts on both linear DNA and plasmid DNA, each having three recognition sites, the restriction enzyme will generate 3 fragments from plasmid DNA (as it is circular) and 4 fragments from linear DNA.
5 Marks
